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KMID : 1134820170460010018
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 1 p.18 ~ p.25
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Antioxidant and Anti-Inflammatory Activities of Crude Extract and Solvent Fractions of Allium hookeri
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Lee Yong-Bum
Ham Young-Min
Yoon Seon-A
Oh Dae-Ju
Song Sang-Mok
Hong In-Choel
Lee Si-Taek
Hyun Ho-Bong
Kim Chang-Suk
Yoon Weon-Jong
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Abstract
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This study describes the preliminary evaluation of antioxidant and anti-inflammatory activities of Allium hookeri. A. hookeri was extracted using crude extract and then fractionated sequentially with n-hexane, CH2Cl2, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, we first examined the inhibitory effect of A. hookeri extracts on production of oxidant stresses (2,2-diphenyl-1-picrylhydrazyl, xanthine oxidase, and superoxide). In addition, we examined the inhibitory effects of A. hookeri on production of pro-inflammatory factors (nitric oxide, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2) in murine macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Of the sequential solvent fractions of A. hookeri, EtOAc fractions showed decreased production of oxidant stresses, and CH2Cl2 and EtOAc fractions of A. hookeri inhibited production of pro-inflammatory factors. EtOAc fraction inhibited production of pro-inflammatory cytokines (interleukin-6 and -1β). These results suggest that A. hookeri has significant effects on oxidant stresses and pro-inflammatory factors and is a possible antioxidant and anti-inflammatory therapeutic and preventive material.
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KEYWORD
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Allium hookeri, pro-inflammatory factors, pro-inflammatory cytokines, lipopolysaccharide, RAW 264.7 cells
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